14-3-3 epsilon antibody
Cat.#: S1560
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P62258
Mol Weight: 29.18kDa
Size: 100ul

Description
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq]

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:500-1:3000

Species Reactivity
Human

Other species have not been tested

Products Data 

 

Sample (30ug of whole cell lysate)
A: H1299
B: Hela S3
12% SDS PAGE
diluted at 1:5000
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using 14-3-3 epsilon antibody at 1:500 dilution.

Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 225 of Human YWHAE

Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.

Alternative Names
YWHAE antibody, 14-3-3E antibody, FLJ45465 antibody, FLJ53559 antibody, KCIP-1 antibody, MDCR antibody, MDS antibody, 14-3-3 protein epsilon antibody, antibody