14-3-3 theta(YWHAQ)antibody
Cat.#: 3887-1
Rabbit Monoclonal Antibody
Clone ID: EPR5123
Swiss Prot: P27348
Mol Weight: 28kDa
Size: 100ul

Description
14-3-3 theta belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and it is 99% identical to the mouse and rat orthologs. 14-3-3 theta is upregulated in patients with amyotrophic lateral sclerosis (1).

Recommended Applications
WB, ICC, FC

Applications and Recommended Dilution Factors
WB: 1:10,000 - 50,000
ICC: 1:50 - 100
FC: 1:500 - 1,000

Species Reactivity
Human, Mouse, Rat

Species cross-reactivity is based on WB analysis

Products Data

 

Western blot analysis on (A) HeLa, (B) 293T, (C) A549, and (D) A431 cell lysates using anti-14-3-3 theta RabMAb (cat. #3887-1).
Flow cytometric analysis of permeablized A549 cells using anti-14-3-3 theta RabMAb (red) (catalog # 3887-1) or a rabbit IgG (negative) (green). 

Specificity
A synthetic peptide corresponding to residues on the N-terminus in human 14-3-3 theta was used as an immunogen.

Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
YWHAQ antibody, 14-3-3 antibody, 1C5 antibody, HS1 antibody, 14-3-3 protein theta antibody, 14-3-3 protein T-cell antibody, 14-3-3 protein tau antibody, Protein HS1 antibody

Description References
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.