产地：usa
货号：L2524
Lyticase from Arthrobacter luteus
CAS号: 37340-57-1
Application
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
相关类型 Application Index, Carbohydrate Hydrolysis, Carbohydrate hydrolysis & PTM analysis, Cell Dissociation and Cell Lysis, Cell Lysis Reagents,
Cell Lysis and Protein Extraction Reagents, Enzymatic Cell Lysis and Protoplast Preparation, Enzymes, Enzymes for Cell Lysis, Enzymes, Inhibitors, and Substrates, Lysing Enzymes, Lysing Enzymes and Protoplast Preparation, Molecular Biology, Molecular Biology Enzymes, Native Protein Sample Preparation, Signal Transduction Research Tools, Yeast
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Quality Level PREMIUM
form lyophilized powder
composition Protein, ≥20% biuret 
storage temp. −20°C
Unit Definition
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Physical form
Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers
本产品仅用于生命科学研究实验。