产地：oxoid
货号：CM0331
名称：Columbia Blood Agar Base
Description
Traditionally, blood agar bases have been either casein hydrolysate or meat infusion media. The advantage of the first lies in the rapid production of large colonies, and of the second in clearly defined zones of haemolysis and good colonial differentiation.
Columbia Agar Base (CM0331) ( Ellner et al.1 ) combines the virtues of both these types of media to give an improved all-round performance.
This new base has shown versatility and superior performance in several applications.
Brucella
To prepare a selective medium add Brucella Selective Supplement SR0083 to 500ml of sterile, molten Columbia Blood Agar Base (CM0331), containing 5-10% v/v inactivated horse serum and 1% w/v dextrose2,3.Columbia Blood Agar Base
Campylobacter and Helicobacter
To prepare a selective medium add:
Campylobacter Selective Supplement (Skirrow) SR00694
or Campylobacter Selective Supplement (Butzler) SR00855,6
or Modified Butzler (ISO) Selective Supplement SR0214
or Campylobacter Selective Supplement (Blaser-Wang) SR00987,8,9
or Helicobacter pylori Supplement SR0147 to 500 ml of sterile, molten Columbia Agar Base (CM0331) containing Campylobacter Growth Supplement SR023210 and 5-7% v/v horse or sheep blood (SR0048, SR0050 or SR0051).Columbia Blood Agar Base
Egg Yolk Emulsion Agar made using Oxoid Columbia Agar Base (CM0331) and Egg Yolk Emulsion SR0047 has been shown to be a satisfactory isolation medium for Helicobacter pylori11.
Gram-positive cocci
see Staph/Strep Selective Supplement SR0070
see Modified CNA Selective Supplement SR0176
see Streptococcus Selective Supplement SR0126
Gardnerella
see Gardnerella vaginalis Selective Supplement SR0119
Directions
Add 39g to 1 litre of distilled water. Boil to dissolve the medium completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and add 5% sterile defibrinated blood.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
Other applications
Elek Test
Columbia Agar Base (CM0331) with added sterile serum provides an efficient Corynebacterium diphtheriae virulence test medium. After following the established technique, lines of toxin-antitoxin precipitation are clearly visible in 48 hours.
Nagler Test
The addition of 5ml Fildes Extract SR0046 and 10ml of Egg Yolk Emulsion SR0047 to 100ml of sterile, molten Columbia Blood Agar Base (CM0331) will provide a diagnostic medium for Clostridium perfringens, when used with Clostridium perfringens antitoxin (Nagler reaction) and neomycin (100-125m/ml)12.
Reversed CAMP Test for Clostridium perfringens
The reversed CAMP test13 is a highly sensitive and specific test for Clostridium perfringens which may be used as an alternative to the Nagler test.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.
Technique
Inoculate the culture suspected to be Clostridium perfringens in a straight line across a plate of Columbia sheep blood agar. Streak an overnight (or older) culture of Streptococcus agalactiae known to produce the CAMP factor at right angles to the first inoculation taking care that the lines do not touch. Incubate anaerobically at 35-37°C for 18-24 hours.
A positive reverse CAMP test is indicated by the formation of an ``arrowhead’’ of haemolysis between the lines of the Clostridium perfringens and Streptococcus agalactiae growth.Columbia Blood Agar Base