Replaces Prod. #: ALX-630-081
Increases cytosolic Ca2+ levels and stimulates platelet aggregation. Activates phospholipase C (PLC). The B oligomer is a subunit of pertussis toxin responsible for binding of the holotoxin to eukaryotic cell surfaces, facilitating entry of the A promoter into receptive cells. Lacks intrinsic ADP-ribosyltransferase activity but still induces several responses in sensitive cells, e.g. human t-cell mitogenesis, human platelet aggregation and insulin-like action in rat adipocytes.
Product Specification
Alternative Name: Islet-activating protein B oligomer, PTX B oligomer
Formulation: Lyophilized.
Source/Host: From Bordetella pertussis.
Long Term Storage: +4°C
Use/Stability: Store at 4°C prior to and following reconstitution. The lyophilized preparation will remain active for at least one year. After reconstitution with sterile water this prodcut is stable for at least four weeks if stored at 4°C.
Hazard: TOXIC.
Handling: Do not freeze.
Do not sterile filter, as this will result in loss of material. Do not vortex.
Miscellaneous/General: The B oligomer (S-2, S-3, S-4, and S-5) of pertussis toxin attaches the native toxin to eukaryotic cell surfaces and facilitates the translocation of the A protomer. It does not possess any intrinsic ADP-ribosyltransferase activity. It does elicit several physiologic responses including human T cell mitogenesis, human platelet aggregation and insulin-like action in rat adipocytes.