Product Specification
Purity Detail: Purified.
Formulation: Liquid. In PBS containing 0.1% sodium azide.
Clone: FK1
Isotype: IgM
Immunogen: Poly-ubiquitinylated lysozyme.
Source/Host: From mouse
Specificity: Recognizes polyubiquitinylated conjugates. Species independent. Does not cross-react with mono-ubiquitinylated proteins or free ubiquitin.
Application: ELISA
Immunohistochemistry
Western Blot: (1:1’000)
Optimal conditions must be determined individually for each application.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: +4°C
Handling: Do not freeze.
Store unopened vial at 4°C until required for use. Store diluted antibody at 2-4°C and use within 1 month.
The hybridoma secreting MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Polyubiquitinylated Conjugates (FK1) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise only polyubiquitinylated proteins and not monoubiquitinylated proteins or free ubiquitin.