Product Specification
Alternative Name: Ub-H
MW: 8.5kDa
Purity: ≥95% (HPLC)
Sequence: Recombinant Ubiquitin (corresponding to UniProt sequence P62988). The C-terminal glycine carboxyl is synthetically modified to an aldehyde.
Formulation: Liquid. Aqueous solution containing 0.15M HCl.
Specific Activity: Ki=2.5nM vs. UCH isopeptidase-T.
Quality Control: Ubiquitin Aldehyde (recombinant) activity was confirmed by its inhibition of UCH-L1 (PGP9.5) (human) (recombinant) (GST) (Prod. No. BML-UW9305) deubiquitinylation of Ubiquitin-AMC (Prod. No. BML-SE211).
Application: Ubiquitin Aldehyde (recombinant) is useful in the stabilisation of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ubiquitin Aldehyde (recombinant) can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5µM.
Co-crystallisation of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme:substrate interactions.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: +4°C
Use/Stability: Stable for at least two months when stored at 4°C.
Handling: Soluble and stable in aqueous solution at pH <7.0.
Avoid presence of amino-containing compounds.
Do not lyophilize.
Do not neutralise until immediateley prior to use.
Background / Technical Information: Typical assay set-up:Substrate concentration: 0.01-1.0μM.  Enzyme concentration, UCH-L1 (PGP9.5) (human) (recombinant) (GST) (BML-UW9305): 10-100nM.  Inhibitor concentration: 0.01-1.0µM.  Release of AMC fluorescence by DUB enzymes can be monitored using 380nm excitation and 460nm emission wavelengths.Please click here for the comprehensive product datasheet.
Replaces Prod. #: ALX-200-603
Synthetically modified recombinant ubiquitin. The modification of the C-terminal glycine carboxyl into an aldehyde results in a potent, highly specific inhibitor of all ubiquitin deconjugating enzymes, including ubiquitin C-terminal hydrolases (UCHs), ubiquitin-specific proteases (USPs) and deubiquitinylating enzymes (DUBs).