货号：74903
产地：qiagen
QIAshredder
简单快速均质化细胞和组织的裂解液
代替针筒抽吸的均质化步骤
可以减少样品的消耗
防止样品间的交叉污染
可以去除不溶性沉淀碎片和降低粘稠度
Principle
The QIAshredder homogenizer consists of a unique biopolymer-shredding system in a microcentrifuge spin-column format. Isolation of total or poly A+ RNA from cell or tissue lysates requires homogenization to reduce viscosity caused by high-molecular-weight cellular components and cell debris. Traditional methods use syringes and needles, which are tedious, inconvenient, and hazardous to handle. QIAshredder homogenizers replace these homogenization methods with a fast and simple centrifugation step. In general, similar yields and RNA quality are obtained for cell lysates as with rotorCstator homogenization .
Procedure
Up to 700 µl of cell or tissue lysate can be homogenized by pipetting or decanting the lysate into a QIAshredder homogenizer and centrifuging for 2 minutes at full speed in a microcentrifuge. The homogenized lysate is ready to use in nucleic acid isolation procedures.
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