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BML-PW0530-0100 100 µl
BML-PW0530-0025 25 µl
Product Specification
Alternative Name: 26S Proteasome subunit ATPase 1
Purity Detail: Partially purified by salt precipitation.
Formulation: Liquid. In PBS containing 0.01% sodium azide.
Immunogen: Synthetic peptide corresponding to aa 368-383 of human proteasome 19S (Rpt2/S4 subunit).
Source/Host: From rabbit.
Specificity: Recognizes human proteasome 19S (Rpt2/S4 subunit).
Application: Western Blot (1:1'000)
Optimal conditions must be determined individually for each application.
Shipping: SHIPPED ON BLUE ICE
Short Term Storage: +4°C
Long Term Storage: -20°C
Use/Stability: Stable for at least 1 year after receipt when stored at -20°C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4°C, do not freeze and use within 1 month.
Handling: After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.  
Miscellaneous/General: The 19S regulatory complex consists of a ‘base’ sub-complex, composed of a hetero-hexameric ring of AAA-ATPases that not only function as the gate-opener to the 20S proteasome but also, in conjunction with two other high-molecular-mass non-ATPase subunits (Rpn2/S1 and Rpn1/S2), as a platform for protein unfolding. The ‘lid’ sub-complex of the 19S complex, composed of non-ATPase subunits is involved in the binding and deubiquitinylation of proteasome targeted ubiquitin conjugated proteins and assists in the transport of substrates through the ‘base’ and into the 20S catalytic core.