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BML-PW0600-0100 100 µl
BML-PW0600-0025 25 µl
Modification of proteins by addition of K63-linked polyubiquitin chains is implicated in a variety of cellular events, including DNA repair, signal transduction and receptor endocytosis.
A monoclonal antibody (mAb) has been generated that specifically recognizes K63-linked polyubiquitin chains, but not any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitin chain or monoubiquitin. The specificity of this K63-linked ubiquitinspecific monoclonal antibody was demonstrated using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in both cell lysates and intact cells.
The hybridoma secreting the antibody [clone HWA4C4] was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a synthetic peptide encompassing the ubiquitin K63-isopeptide motif. Immunoglobulin was purified from tissue culture supernatant by ion exchange chromatography.
Product Specification
Concentration: 1mg/ml
Formulation: Liquid. In PBS containing 0.09% sodium azide.
Clone: HWA4C4
Isotype: Mouse IgG2a
Immunogen: Synthetic peptide containing the ubiquitin K63-isopeptide motif.
Specificity: Recognizes K63-linked polyubiquitin in wide range of species. Does not cross-react with any other isopeptide-linked (K6, K11, K27, K29, K33, or K48) polyubiquitinylated species.
Application: ELISA
Immunocytochemistry: (1:100)
Immunohistochemistry: (1:100)
Western Blot: (1:500 - 1:1’000)
Optimal conditions must be determined individually for each application.
Shipping: SHIPPED ON BLUE ICE
Long Term Storage: -20°C  
Handling: After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.