| Catalogue Number | ANT-283 |
| Synonyms | B70, B7-2, LAB72, CD28LG2, FUN-1, BU63. |
| Type | Rat Anti Mouse Monoclonal. |
| Introduction | CD86 type I membrane protein is a member of the immunoglobulin superfamily which is expressed by antigen-presenting cells, and acts as the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. CD86 binding with CD28 protein is a co-stimulatory signal for initiation of the T-cell. CD86 binding with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. |
| Clone | mB7-2. |
| Immunogen | Purified mouse LPS-activated B cells. |
| Ig Subclass | Rat IgG2a. |
| Purification Method | Protein-A. |
| Protein concentration | 1mg/ml in PBS (after reconstitution). |
| Storage Procedures | Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. |
| Reconstitution | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. |
| Antibody Properties | Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking T cell activation should be determined by the investigator. |
| Available Conjugates | This antibody is only available conjugated to Biotin and FITC. |
| Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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