Analysis Note
Protein determined by biuret.
Application
For the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters
α-glucosidase is used for the determination of α-amylase and the synthesis of various 1′-O-sucrose and 1-O-fructose esters. It was also used in the measurement of glycosidase inhibition.[2]
Biochem/physiol Actions
Hydrolysis of terminal, non-reducing 1→4-linked D-glucose residues with release of D-glucose.
α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus[1].
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
包装
100 units in glass bottle
Sold on basis of p-nitrophenyl α-D-glucoside units.
1000 units in poly bottle
Unit Definition
One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.
性质
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