PubChem Substance ID 24894305

产品描述

Biochem/physiol Actions

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution

Protect from light.

包装

1, 5, 10, 50 mg in glass bottle

Application

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Suitable for

• DNA staining in agarose gels

• analysis of changes in DNA during apoptosis

• detection of mycoplasma

• photofootprinting of DNA

• immunofluorescent staining of cells

DAPI has been used:-

• in rapid monitoring of microbial contamination[2] 

• in chromosomal banding technique[3]

• in detection of apoptotic cells[4]

• in fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA[5]

• to stain mature pollen grains(0.5 mg/ml)[6] 

General description

DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.

性质

Related CategoriesApoptosis Detection, Apoptosis and Cell Cycle, Fluorescent, Nucleic Acid Detection Substrates and Reagents, Reagents,

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Quality Level  PREMIUM

grade  for molecular biology

InChI Key  FPNZBYLXNYPRLR-UHFFFAOYSA-N

assay  ≥98% (HPLC and TLC)

form  powder

solubility  H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)

 PBS: insoluble

absorption  ε1mM/263 nm, H2O 30

fluorescence  λex 340 nm; λem 488 nm (nur DAPI)

 λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

suitability  suitable for fluorescence

storage temp.  2-8°C

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