胶原蛋白酶 来源于溶组织梭菌

Collagenase from Clostridium histolyticum

release of physiologically active rat epididymal adipocytes tested, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

安全技术说明书

别名: 梭菌肽酶 A

•  EINECS 编号 232-582-9

   

•  Enzyme Commission (EC) Number 3.4.24.3  ( BRENDA | IUBMB )

•  MDL number MFCD00130830

• POPULAR DOCUMENTS:  PRODUCT INFORMATION SHEET (PDF)  |  SPECIFICATION SHEET (PDF)

  产品描述

   

  Biochem/physiol Actions

  Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

  Collagenase is activated by four gram atom calcium, per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue.^[2] Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.^[4]

  Caution

  As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. The product retains 100% activity over 7 hours when held on ice.

  包装

  100, 500 mg in poly bottle

  Preparation Note

  Also contains clostripain, nonspecific neutral protease, and tryptic activities.

  Unit Definition

  One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

  Application

  Collagenase may be used: ^[3][1]
  • for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)
  • for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.
  • in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes
  • for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue

  General description

  Clostridium histolyticum is a pathogenic clostridium that produces collagenase. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.^[1]

  性质

   

  Related Categories	3.4.x.x Peptidases, 3.x.x.x Hydrolases, Apoptosis and Cell Cycle, Application Index, Cell Dissociation, 更多...
  form	powder
  suitability	release of physiologically active rat epididymal adipocytes tested
  Featured Industry	Diagnostic Assay Manufacturing
  shipped in	wet ice
  storage temp.	−20°C

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