| 应用 |
A structural analog to Tris buffer. |
| 产品介绍 |
useful pH range 6.8 - 8.2 pKa (25 °C) 7.5 |
| 备注 |
Hydrogen ion buffer for biological research1; Suitable buffer for epidermal cell growth at pH 7.4-7.9 where additional buffering capacity is required |
| 生化机理 |
TES is a structural analog to Tris buffer. It is one of the ethanesulfonic acid series of biological buffers developed by Good et al. Good’s buffer criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range, and easily synthesized. TES probably exists in solution as a zwitterion. Because the pKa is 7.4 (physiological pH) this buffer has considerable potential for a variety of biological applications. A buffer using TES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH. Alternatively, solutions of equimolar TES free acid and TES sodium can be mixed to attain a buffer of the desired pH. |
| 别名 |
2-[[三(羟甲基)甲基]氨基]乙磺酸;N-三(羟甲基)甲基-2-氨基乙烷磺酸;TES 游离酸;2-[(三(羟甲基)甲基)氨基]-1-乙磺酸;3-[(羟甲基)甲基胺]乙烷磺酸2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid;N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid;TES free acid |
