DNA酶I 来源于牛胰腺(重组)-分析测试百科网

DNA酶I 来源于牛胰腺(重组)

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品牌：阿拉丁
厂商性质：生产商
产地类别：国产
产地：上海
货期：现货
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EC号 232-667-0 MDL号 MFCD00130918 属性敏感性易潮湿存贮条件储存温度2-8℃ //decorateTable('product-attribute-specs-table') 描述应用用于从蛋白质样品

上海阿拉丁生化科技股份有限公司

上海阿拉丁生化科技股份有限公司

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货号	CAS号	操作
D128586-10KU	9003-98-9	询底价

耗材描述

EC号 232-667-0
MDL号 MFCD00130918

属性

敏感性	易潮湿
存贮条件	储存温度2-8℃

描述

应用	用于从蛋白质样品中除去 DNA。 Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a two-dimensional zymogram analysis of nucleases in Bacillus subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major groove-binding drugs and intercalators on the DNA association of minor groove-binding proteins RecA and deoxyribonuclease I.
备注	Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases, and proteases. A lyophilized powder containing glycine as a stabilizer.
生化机理	DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. The pH optimum is found to be between 7 and 8. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS)6 and actin7 are known to inhibit the enzyme activity.
别名	脱氧核糖核酸酶 I;脱氧核糖核酸 5′-寡核苷酸-水解酶Deoxyribonucleate 5′-oligonucleotido-hydrolase;DNase I

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