胰蛋白酶 来源于牛胰腺(TPCK处理,辐射)
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EC号 232-650-8 MDL号 MFCD00082094 属性 存贮条件 储存温度 -20°C //decorateTable('product-attribute-specs-table') 描述 应用 Trypsin is a s
| 货号 | CAS号 | 操作 |
|---|---|---|
| T128773-100mg | 9002-07-7 | 询底价 |
耗材描述
- EC号 232-650-8
- MDL号 MFCD00082094
属性
| 存贮条件 | 储存温度 -20°C |
描述
| 应用 | Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins |
| 产品介绍 | Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE. |
| 备注 | Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity according to Kostka, V., and Carpenter, F.H.: JBC, 239, 1799 (1964), Product T128771, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR 113.53c. Each vial is filled to contain 100 mg. |
| 生化机理 | Trypsin cleaves peptide chains, amides and esters mainly at the carboxyl side of the amino acids lysine or arginine. Trypsin originates in the pancreas in the form of the zymogen trypsinogen. |
| 别名 | ;Peptidyl peptide hydrolase |
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