胃蛋白酶 来源于猪胃
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分子式 分子量 Beilstein号 EC号 232-629-3 MDL号 MFCD00081840 PubChem编号 属性 存贮条件 储存温度-20°C //decorateTable('product-attribute-specs-
| 货号 | CAS号 | 操作 |
|---|---|---|
| P128678-1g | 9001-75-6 | 询底价 |
| P128678-5g | 9001-75-6 | 询底价 |
耗材描述
- 分子式
- 分子量
- Beilstein号
- EC号 232-629-3
- MDL号 MFCD00081840
- PubChem编号
属性
| 存贮条件 | 储存温度-20°C |
描述
| 应用 | 本品能使胃酸作用后凝固的蛋白质分解成胨，但不能进一步使之分解成氨基酸。其消化力以含0.2%～0.4%盐酸(PH=1.6～1.8)是为最强。胃蛋白酶是一种用于消化蛋白质的肽酶，常用于从抗体制备Fab片段。来自猪胃粘膜的胃蛋白酶已被用于水解小鼠的干燥宫颈样本。胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。 |
| 产品介绍 | Pepsin is an acidic protease. Its inactive zymogen precursor, pepsinogen, is produced in the stomach mucosa. There are several pepsins designated A, B, C, and D. Pepsin A, the major component, has a molecular weight of 35,000 daltons and an optimum pH of approximately 1.0 for substrates such as casein or hemoglobin if the substrate is native protein. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is assayed based on the method of Anson J. Gen. Physiol., 22, 79 (1938) using hemoglobin as the substrate. Pepsin is unstable above pH 6. |
| 备注 | Two times crystallized from dilute alcohol. A lyophilized powder. |
| 生化机理 | 优先裂解：P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键 |
| 别名 | 胃液素;胃朊酶;蛋白酵素;酸腈酶;胃酶；胃蛋白酶 A;Pepsin from porcine gastric mucosa; Pepsin A; Pepsin from hog stomach; Pepsini pulvis; Pepsin powder |
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