产品介绍 |
Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique. |
备注 |
Prepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe: Biochem. Prep VIII (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961). Lyophilized in vials. |
别名 |
;3′-Exonuclease Spleen exonuclease Spleen phosphodiesterase |