| 应用 |
Nuclease from Staphylococcus aureus has been used in a study to assess coagulase and heat-resistant strains found in animals. It has also been used in a study to investigate the expression characteristic of two genes in S. aureus that encode two thermostable nucleases. |
| 产品介绍 |
Micrococcal nuclease catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides. It exhibits exo- and endo-5'-phosphodiesterase activities. The enzyme catalyzes preferential endohydrolysis of the RNA and DNA at sites rich in adenylate or uridylate and deoxyadenylate or thymidylate. The enzyme has a molecular weight of 16,807 daltons and is calcium dependent. The pH optimum is 9.2 but varies depending upon the concentration of ionized calcium present. |
| 备注 |
Chromatographically purified to be essentially homogeneous chromatographically and electrophoretically (SDS-PAGE). A lyophilized powder. |
| 生化机理 |
Cleavage of DNA by Nuclease micrococcal is greater at the 5′ sides of A or T nucleotides than at G or C. Hydrolyzes 5′-phosphodiester bonds of DNA. |
| 别名 |
;MNase;Endonuclease micrococcal;Micrococcal endonuclease |
