UFC5010  Millipore

Amicon Ultra-0.5 离心过滤装置

Ultracel-10 regenerated cellulose membrane, 0.5 mL sample volume

别名：配有Ultracel-10滤膜的Amicon Ultra-0.5离心式过滤器

eCl@ss：36100101   NACRES：NB.24

属性

物料

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

质量水平

400

无菌性

non-sterile

产品线

Amicon®

manufacturer/tradename

Amicon® Ultra

参数

0.5 mL sample volume

technique(s)

protein extraction: suitable
protein purification: suitable

长度

4.99 cm (1.96 in.)

直径

10.8 mm

过滤面积

1 cm2

孔径

10 kDa NMWCO

运输

ambient

说明

一般描述

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

应用

• Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms

• Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC

• Desalting, buffer exchange, and protein dialysis

特点和优势

• Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%

• Concentrates 500 μL down to 15 μL

• Concentration factor 25x to 30x

• High sample recovery (greater than 90% of dilute starting solution)

• Fast processing time: 10 to 30 minutes

• Reverse spin capability provides consistent recoveries

外形

色码：透明

法律信息

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany