赛默飞Thermo Acclaim PolarAdvantage Reversed-Phase LC Column
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Sulfonamide-Embedded Reversed-Phase Columns for Separating Polar CompoundsAcclaim® PolarAdvantage (PA) columns feature a
Sulfonamide-Embedded Reversed-Phase Columns for Separating Polar Compounds
Acclaim® PolarAdvantage (PA) columns feature a patented bonding chemistry that incorporates a polar sulfonamide group near the surface of the silica particle. This unique chemistry provides good hydrolytic stability and allows a single column to resolve a wide variety of polar and nonpolar analytes. The Acclaim PA is compatible with 100% aqueous mobile phases and supports LC/MS analysis.
· Low silanol activity for excellent peak shape with basic compounds
· Novel polar-embedded column chemistry
· Compatible with 0–100% organic solvent mobile phases
· High selectivity for hydrophobic aromatic compounds
· Wide range of applications
Conventional C18 phases are not compatible with highly aqueous mobile phases, due to dewetting. The Acclaim PA column by design has a mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negating the problems of dewetting common with conventional reversed-phase columns.
Acclaim PA columns are available both in 3 and 5 μm particle sizes and 4.6, 3.0, and 2.1 mm diameters, with an average pore diameter of 120 Å. Acclaim PA is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC (RSLC) Columns section for more details). Acclaim PA may be used for LC/MS applications.
Applications
The Acclaim PA column can be used in a wide range of applications in pharmaceutical, environmental, food testing, and product-quality testing. These columns also give efficient separations with good peak shapes for compounds of life science (nucleic acid and bases) and pharmaceutical interest (sulfonamides) using highly aqueous mobile phases.
| Acclaim PolarAdvantage: Rugged Polar-Embedded Reversed-Phase Columns for the Analysis of Polar and Nonpolar Compounds Specifications | |
|---|---|
| Surface coverage | 2.7 μmol/m2 |
| End-capped | Yes |
| Metal impurity (Na, Fe, Al) | <10.0 ppm Total Metal <3.0 ppm Individual Metal |
| Pore volume | 1.0 mL/g |
| Average pore diameter | 120Å |
| Surface area | 300 m2/g |
| pH range | 2–8 |
| Temperature | <60°C |
| Starting material | Ultrapure silica |
| Particle size | 3 and 5µ |
| Particle shape | Spherical |
| Particle size distribution (40/90) | <1.3 |
| Total carbon content | 17% |
Fat-soluble vitamins and carotenoids in a vitamin tablet.
Loratidine and pseudo-ephedrine in a time-release tablet.
LC separation of nitrosamines, as specified in EPA 8270.
Phospholipids in egg lecithin.
Standard particle sizes are 3 and 5 μm. Available in 2.1, 3.0, and 4.6 mm i.d. Lengths are 50, 100, and 150 mm. Capillary formats are available in 75 and 300 μm, 1.0 mm, and custom diameters, and in 50, 150, or 250 mm lengths. Micro precolumn and nano precolumn cartridges are available in several formats. Guard columns in both 2.0 and 4.3 mm sizes packed with 5 μm particles are recommended. Columns for fast LC are listed in the RSLC section.
| Analytical Columns | |
|---|---|
| Acclaim PA, 3 µm Analytical (2.1 x 50 mm) | 063174 |
| Acclaim PA, 3 µm Analytical (2.1 x 100 mm) | 061316 |
| Acclaim PA, 3 µm Analytical (2.1 x 150 mm) | 061317 |
| Acclaim 120, PA, 3µm Analytical (2.1 x 250mm) | 076215 |
| Acclaim PA, 3µm Analytical, (3.0 x 50mm) | 068972 |
| Acclaim 120, PA, 3µm Analytical (3.0 x 100mm) | 076214 |
| Acclaim PA, 3 µm Analyitcal (3.0 x 150 mm) | 063693 |
| Acclaim PA, 3 µm, Analytical, (3.0 x 250 mm) | 070079 |
| Acclaim PA, 3 µm Analytical (4.6 x 150 mm) | 061318 |
| Acclaim 120, PA, 3µm Analytical (4.6 x 100mm) | 076216 |
| Acclaim PA, 5 µm Analytical (4.6 x 50 mm) | 061319 |
| Acclaim PA, 5 µm Analytical (4.6 x 150 mm) | 061320 |
| Acclaim PA, 5 µm Analytical (4.6 x 250 mm) | 061321 |
| Guard Columns | |
|---|---|
| Acclaim PA, 5µm,2mm Guard Cartridges, (2.1 x 10mm), 2 ea; requires holder 069580 | 069691 |
| Acclaim PA, 5µm Guard Cartridges (3 x 10mm), 2ea, requires holder 069580 | 071983 |
| Acclaim PA, 5 µm Guard Cartridges, (4.6 x 10 mm), 2 ea (use V-2 Holder) | 069698 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | 074188 |
| Acclaim RSLC PA Analytical Columns | |
|---|---|
| Acclaim RSLC PA, 3 µm Analytical (3.0 x 33 mm) | 066274 |
| Acclaim PA, 3µm Analytical, (3.0 x 50mm) | 068972 |
| Acclaim RSLC PA, 3 µm Analytical (3.0 x 75 mm) | 066275 |
| Acclaim PA, 3 µm Analyitcal (3.0 x 150 mm) | 063693 |
| Acclaim RSLC PA, 2.2µm, 2.1 x 50 mm | 072622 |
| Acclaim RSLC PA, 2.2µm, 2.1 x 30 mm | 072621 |
| Acclaim RSLC PA, 2.2µm, 2.1 x 100 mm | 072623 |
| Acclaim RSLC PA, 2.2µm, 2.1 x 150 mm | 072624 |
| Acclaim RSLC PA, 2.2µm Analytical (2.1 x 250mm) | 074813 |
| Acclaim RSLC PA, 2.2µm, 3 x 30mm | 072625 |
| Acclaim RSLC PA, 2.2µm, 3 x 100mm | 072627 |
| Acclaim RSLC PA, 2.2µm, 3 x 50mm | 072626 |
