赛默飞Thermo IonPac NS1 Reversed-Phase LC Column
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Polymeric Reversed-Phase Column Ideal for the Separation of Hydrophobic, Ionizable Compounds
The IonPac®NS1-10μm and NS1-5μm columns are packed with a neutral, macroporous, high-surface-area, ethylvinylbenzene polymer crosslinked with 55% divinylbenzene. This resin makes the NS1 resistant to solvents, acids, and bases, and permits the use of eluent from pH 0 to 14.
Ideal for separation of large molecules that carry localized charges, such as surfactants
Compatible with acids, bases, and solvent from pH 0 to 14
Can also be used for traditional polymeric reversed-phase applications
Utilize ion-pair chromatography for difficult separations
The IonPac NS1 column is the column of choice for routine ion-pair chromatography or ion-suppression separations using theAMMS®ICE 300 suppressor. The NS1 is also used with mobile phase ion chromatography (MPIC®), a technique especially suited to large molecules that carry localized charges (e.g., surfactants), as well as other ions that are not amenable to separation by ion-exchange chromatography.
Key Applications
The IonPac NS1 column can be used for the separation of high MW aliphatic carboxylic acids, alkyl and aromatic sulfates and sulfonates, quaternary ammonium ions, water-soluble vitamins, sulfur oxides, metal cyanide complexes, phenols, alkanolamines.
| IonPac NS1 Polymeric Reversed-Phase ColumnSpecifications | |
|---|---|
| Dimensions | IonPac NS1-10µm Analytical Column: 4×250 mm IonPac NS1-10µm Guard Column: 4 × 35 mm IonPac NS1-5µm Analytical Column: 4 × 150 mm |
| Maximum operating pressure | <4000 psi (<27.6 MPa) |
| Typical operating conditions | Gradient: 1.0 mL/min Recommended Operating Temperature: Ambient Recommended Flow Rate: 1.0 mL/min Typical Ion-Pairing Reagents: For anions: ammonium and tetramethyl-, tetraethyl-, tetrapropyl-, and tetrabutyl-ammonium hydroxides. For cations: hydrochloric, perchloric, and perfluorocarboxylic acids; pentane-, hexande-, heptane-, and octanesulfonic acids. |
| Mobile phase compatibility | pH 0–14; 1–100% HPLC solvents |
| Resin composition | Bead Diameter: 10 µm (4×250 mm), 5 µm (4×150 mm), macroporous ethylvinylbenzene/divinylbenzene resin Cross-Linking (%DVB): 55% Hydrophobicity: High |
| Capacity | 60 µeq (2×250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
Separation of aliphatic sulfonic acids using tetrabutylammonium ion as the ion-pair reagent.
Determination of aliphatic quaternary ammonium ions using nonafluoropentanoate ion as the ion-pair reagent with an acetonitrile gradient.
Using the IonPac NS1 Column Determination of aliphatic carboxylic acids.
Separation of Alkanolamines on the IonPac NS1 Column Using Ion Pairing with Suppressed Conductivity Detection.
| Analytical Columns | |
|---|---|
| IonPac NS1-10 µm Analytical Column (4 x 250 mm) | 035321 |
| IonPac NS1-5 µm Analytical Column (4 x 150 mm) | 039568 |
| IonPac NS1 10 µm Analytical Column (2 x 250 mm) | SP4354 |
| Guard Columns | |
|---|---|
| IonPac NG1-10 µm Guard Column (4 x 35 mm) | 039567 |
| IonPac NG1-10 µm Guard Column (2 x 50 mm) | SP4356 |
| DIONEX IONPAC NG-1 CAPILLARY GUARD (0.4X50MM) | SP6944 |