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MQAE

CAS No. : 162558-52-3

MCE 国际站：MQAE

产品活性：MQAE 是一种紫外激发的新型氯离子荧光探针。MQAE 以溴离子做配体，可用于检测氯离子活性。MQAE 的荧光强度会随着胞内氯离子浓度升高而降低。氯离子浓度低于 50 nM 时，MQAE 具有 pH 独立的荧光强度。MQAE 的水溶性和细胞渗透性高，可适用于共聚焦显微镜与流式细胞仪等荧光检测。其荧光波长：Ex/Em=350/460 nm。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Preparation of MQAE working solution
1.1 Preparation of the stock solution
Dissolve 1mg MQAE in 0.3066mL DMSO to obtain 10 mM of MQAE.
Note: It is recommended to store the stock solution at -20 °C -80 °C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of MQAE working solution
Dilute the stock solution in Krebs-hepes buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl2, 1 mM MgCl2, 16 mM glucose, pH 7.4) to obtain 5-10 μM of MQAE working solution.
Note: Please adjust the concentration of MQAE working solution according to the actual situation.

Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of MQAE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS.

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