MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
CAS No. : 217075-36-0
MCE 国际站:BODIPY 581/591 C11
产品活性:BODIPY 581/591 C11 是一种 BODIPY 氟硼荧衍生物,具有良好的光稳定性、低荧光伪影特质。BODIPY 581/591 C11 常用于活细胞内脂质过氧化和抗氧化性能的研究,或与羟基自由基反应、检测铁死亡 (ferroptosis)。BODIPY 581/591 C11 发射波长为 591 nm (还原型),或红移至 510 nm (氧化型);激发波长分别为 581 nm (还原型)、500 nm (氧化型)。
研究领域:Others | Apoptosis
作用靶点:Fluorescent Dye | Ferroptosis
In Vitro: Preparation of the stock solution
Dissolve 1mg BODIPY 581/591 C11 in 0.1983 mL DMSO to obtain 10 mM of BODIPY 581/591 C11.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of BODIPY 581/591 C11 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 2-10 μM of BODIPY 581/591 C11 working solution.
Note: Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation.
Cell staining
2.1 Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of BODIPY 581/591 C11 working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of BODIPY 581/591 C11 working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
相关产品:MeOSuc-Ala-Ala-Pro-Val-pNA | BDP 558/568 amine | AC-green | Naphthalene-1,2-dicarbaldehyde | Rhodamine 800 | Vari Fluor 532 SE | Acridine Orange hydrochloride | N-(7-Nitrobenzofurazan-4-yl)phallacidin | Sorafenib-d4 | Biotin-11-UTP | BODIPY FL SSE | Lapatinib ditosylate | 1,2-Diamino-3,4-ethylenedioxybenzene | Sulfo-Cyanine5.5 NHS ester tripotassium | Ciclopirox olamine | BODIPY 505/515 | Curcumin | L-Glutamine-d5 | 3-Acetylumbelliferyl β-D-Glucopyranoside | Ac-DNLD-AMC | Mucicarmine | 7-Aminocoumarin | HDAC-IN-48 | NEP | Sulfo-cyanine3 azide sodium | Dihydrorhodamine 123 | Allophycocyanin | (S)-L-Cystine-15N2 | N-Acryloyl-1-pyrenebutylamine | TSPP tetrasodium
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物 | 寡核苷酸 | 抗体 | 点击化学
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Fluorescent Dye | PROTAC | Isotope-Labeled Compounds | Oligonucleotides | Antibodies | Click Chemistry
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;
• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。