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Nile Red

CAS No. : 7385-67-3

MCE 国际站：Nile Red

产品活性：尼罗红 (Nile blue oxazone) 是一种亲脂染色。尼罗红具有环境敏感性荧光。尼罗红在富脂环境中荧光强烈，而在水介质中荧光微弱。尼罗红是荧光显微镜和流式细胞荧光法检测细胞内脂滴的极好的重要染色剂。尼罗红将细胞内脂滴染成红色。荧光波长559/635 nm。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: 1. Preparation of Phalloidin-TRITC working solution
1.1Preparation of the stock solution
Dissolve Phalloidin-TRITC in Methanol to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Phalloidin-TRITC working solution
Dilute the stock solution in serum-free cell culture medium to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Phalloidin-TRITC working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
b.Add 1 mL of working solution, and then incubate at room temperature for 30-60 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a.Culture adherent cells on sterile coverslips.
b.Remove the coverslip from the medium and aspirate excess medium.
c.Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 30-60 minutes.
d.Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

In Vivo: When Nile red-stained Caenorhabditis elegans is viewed for green fluorescence, discrete lipid bodies can be observed throughout the intestine and other tissues either in clusters or evenly dispersed, depending on the animal's genotype or experimental treatment.

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