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DASPEI

CAS No. : 3785-01-1

MCE 国际站：DASPEI

产品活性：DASPEI 是一种 Stokes 位移较大的阳离子苯乙烯基线粒体染料。DASPEI 的激发和发射波长在 550/573 nm，荧光寿命强，灵敏度高。DASPEI 可以对活细胞中的线粒体进行染色，具有良好的标记性，也可独立于神经元活性用于突触前神经末节的染色。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Preparation of DASPEI working solution
1) Preparation of the stock solution
Dissolve 1 mg of DASPEI in 0.2016 mL of DMSO to obtain 10 mM of DASPEI
Note: It is recommended to store the stock solution at -20 ℃ -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
2) Preparation of DASPEI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5-1 mM of DASPEI working solution.
Note: Please adjust the concentration of DASPEI working solution according to the actual situation.


Cell staining
1) For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2) Add 1 mL of DASPEI working solution, and then incubate at room temperature for 10-30 minutes.
3) Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
4) Wash twice with PBS, 5 minutes each time.
5) Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

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