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细胞凋亡与坏死检测试剂盒

MCE 国际站：细胞凋亡与坏死检测试剂盒

MCE 细胞凋亡与坏死检测试剂盒采用 Hoechst 33342 和碘化丙啶（Propidium Iodide, PI）双染的方法快速检测细胞凋亡与细胞坏死。

Description & Advantages:

MCE Apoptosis and Necrosis Assay Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. This kit contains two ready-to-use dyes bound to DNA. Hoechst 33342, a type of blue fluorescence dye (excitation/emission maxima ≈ 352/461 nm when bound to DNA), stains the condensed chromatin in apoptotic cells more brightly than the chromatin in normal cells. Propidium Iodide (PI) is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids. The simultaneous use of these two dyes makes it possible to distinguish normal, apoptotic, and dead cell populations by flow cytometer or fluorescence microscope. Normal cells show weak blue fluorescence + weak red fluorescence, apoptotic cells show strong blue fluorescence + weak red fluorescence, necrotic cells show strong blue fluorescence + strong red fluorescence.

This kit takes only 20-30 minutes to complete one-step staining and there is no need for dilution or preparation of other solutions when detected by flow cytometer. Each kit contains enough reagents for 100 samples.

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