MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

TWEAK/TNFSF12 Protein, Human/Cynomolgus (HEK293, Fc)

MCE 国际站：TWEAK/TNFSF12 Protein, Human/Cynomolgus (HEK293, Fc)

TWEAK 蛋白是细胞因子肿瘤坏死因子样细胞凋亡弱诱导因子，属于肿瘤坏死因子 (TNF) 超家族。TWEAK 蛋白与 FN14 和 TNRFSF12/APO3 结合，是细胞凋亡的弱诱导因子。TWEAK 蛋白对肿瘤细胞有促凋亡活性，可介导 NF-kappa-B 的激活，促进血管生成和内皮细胞 (ECs) 增殖。TWEAK 蛋白还能增加 IL-6 和 IL-8 的分泌，增强 TNF 和 IL-1 的促炎活性。人的 TWEAK 蛋白 (M1-H249) 是一种 II 型跨膜糖蛋白，含有一个跨膜结构域 (22-42 a.a.)。重组人/食蟹猴 TWEAK/TNFSF12 蛋白 (HEK293, Fc) 是 TWEAK 蛋白的胞外部分 (S97-H249)，由 HEK293 细胞表达，带有 N 端 mFc 标签。

Synonyms: Tumor necrosis factor ligand superfamily member 12; TWEAK; APO3L; DR3LG

Species: Cynomolgus

Source: HEK293

Biological Activity: 1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is typically 2-8 ng/mL.
2. Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A, The EC₅₀ of human Fc-TNFRSF12A is 0.07-0.15 μg/ml.

研究背景：TWEAK Protein refers to the cytokine tumor necrosis factor-like weak inducer of apoptosis. It is a multifunctional cytokine belonging to tumor necrosis factor (TNF) superfamily, acts function by binding TweakR/Fn14 receptor. TWEAK is a cell surface-associated type II transmembrane protein with 2 types protein chain: the membrane form and the secreted or soluble form. The soluble form derives from the membrane form by proteolytic processing. The protein sequences in human and mouse is very different with similarity of 24.79%.
TWEAK binds to FN14 and possibly also to TNRFSF12/APO3, is a weak inducer of apoptosis in some cell types. TWEAK mediates NF-kappa-B activation, promotes angiogenesis and the proliferation of endothelial cells.
TWEAK has multiple biological activities, many of which are associated with immune system development and function.
TWEAK does have pro-apoptotic activity on a select group of human tumor cell lines and on monocytes, while it promotes cell proliferation in human vascular EC and SMC. Furthermore, FGF-2 co-treatment can potentiate TWEAK-stimulated HUVEC proliferation, an effect that may be due to the ability of FGF-2 to up-regulate TweakR/Fn14 gene expression. At the meanwhile TWEAK-TweakR/Fn14 autocrine signaling promotes human microvascular renal EC (HMREC) migration.
TWEAK also plays key role in inflammatory response. TWEAK, stimulates interleukin (IL)-8 secretion in human tumor cell lines, WI-38 fibroblasts and astrocytes. TWEAK also increases IL-6 secretion and ICAM-1 expression in astrocyte cell. Moreover, TWEAK co-incubation could potentiate the pro-inflammatory activities of TNF and IL-1, and concluded that TWEAK could be involved in the pathogenesis of chronic inflammatory diseases.
Above all, TWEAK involves in stimulation of cell growth and angiogenesis, induction of inflammatory cytokines, and under some experimental conditions, stimulation of apoptosis.

蛋白编号：F7HGN4 (S94-H249)

基因 ID：407977

产品分类：细胞因子和生长因子  |  肿瘤坏死因子超家族  |  肿瘤坏死因子配体  |  TWEAK 蛋白

Categories：Cytokines and Growth Factors  |  TNF Superfamily  |  TNF Superfamily Ligands  |  TWEAK Proteins

品牌介绍:
•   MCE (MedChemExpress) 拥有200 多种全球独家化合物库，我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物；
•   50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领；
•   产品种类涵盖各种重组蛋白，多肽，常用试剂盒 ，更有 PROTAC、ADC 等特色产品，广泛应用于新药研发、生命科学等科研项目；
•   提供虚拟筛选，离子通道筛选，代谢组学分析检测分析，药物筛选等专业技术服务；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；
•   专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。