MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务。

LIGHT/TNFSF14 Protein, Human (HEK293, hFC-Myc)

MCE 国际站：LIGHT/TNFSF14 Protein, Human (HEK293, hFC-Myc)

LIGHT蛋白 (CD258; TNFRSF14) 是由活化 T 细胞产生的 II 型跨膜蛋白，是 TNFRSF14/HVEM 配体，属于肿瘤坏死因子 (TNF) 家族。LIGHT 蛋白主要在脾脏中表达，具有膜结合型和可溶性型两种亚型。LIGHT蛋白可以作为肿瘤的免疫检查点分子，并刺激自然杀伤细胞产生干扰素 TFNγ，触发肿瘤凋亡信号。LIGHT 蛋白还参与 LTβR-NIK-p52 NF-κB 的显性通路促进炎症基因表达。此外，LIGHT 蛋白是激活淋巴样细胞的共刺激因子，对疱疹病毒感染有抑制作用。人 LIGHT/TNFSF14 蛋白含有 240 个氨基酸和一个跨膜结构域 (38-58 a.a.)。重组人 LIGHT/TNFSF14 蛋白 (HEK293, hFC-Myc) 是人 LIGHT/TNFSF14 蛋白的胞外部分 (D74-V240)，由 HEK293 细胞表达，具有 N 端 hFc 和 Myc 标记。

Synonyms: Herpes virus entry mediator ligand; HVEM-L; Herpesvirus entry mediator ligand; CD258; HVEML; LIGHT; UNQ391; PRO726;

Species: Human

Source: HEK293

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized TNFRSF14 at 0.5 μg/mL can bind TNFSF14 at 0.1525-20000 ng/mL, the EC₅₀ is 3.488-13.38 ng/mL.

研究背景：LIGHT/TNFSF14 is a type II transmembrane protein produced by activated T cells, belongs to tumor necrosis factor (TNF) family. LIGHT/TNFSF14 is a TNFRSF14/HVEM (herpesvirus entry mediator) ligand, engages the receptor for the LTalphabeta heterotrimer but does not form complexes with either secreted lymphotoxin alpha (LTalpha) or LTbeta.
LIGHT/TNFSF14 is predominantly expressed in the spleen but also found in the brain. It is weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
LIGHT/TNFSF14 has a transmemberane, thus it can be leaved into 2 chains: membrane form and soluble form. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
In tumor immunology, TNFSF14/LIGHT also serves as a novel immune checkpoint molecule for glioblastoma multiforme (GBM), as well as lung carcinoma, breast carcinoma, cervical cancer, and prostate cancer. TNFSF14/LIGHT can stimulate NK cells to produce IFNγ via nuclear factor-κB (NFκB) RelA/p50 signaling. TNFSF14/LIGHT sustains the function of CD8⁺ effector T cells, trigger apoptosis of various tumor cells.
In cell signaling, TNFSF14/LIGHT binds to lymphotoxin-β receptor (LTβR) and HVEM for activating both of them, and disrupts the HVEM-BTLA complex in surface-bound form, and facilitates HVEM-BTLA complex formation in the soluble form.
TNFSF14/LIGHT promotes an inflammatory esophageal fibroblast in vitro via a LTβR-NIK-p52 NF-κB dominant pathway with promoting inflammatory gene expression and down-regulating homeostatic factors including WNTs, BMPs and type 3 semaphorins.
Beside that, TNFSF14/LIGHT protein is a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. TNFSF14/LIGHT also prevents tumor necrosis factor alpha mediated apoptosis in primary hepatocyte.

蛋白编号：O43557 (D74-V240)

基因 ID：8740

产品分类：细胞因子和生长因子  |  肿瘤坏死因子超家族  |  肿瘤坏死因子配体  |  LIGHT 蛋白

Categories：Cytokines and Growth Factors  |  TNF Superfamily  |  TNF Superfamily Ligands  |  LIGHT Proteins

品牌介绍:
•   MCE (MedChemExpress) 拥有200 多种全球独家化合物库，我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物；
•   50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领；
•   产品种类涵盖各种重组蛋白，多肽，常用试剂盒 ，更有 PROTAC、ADC 等特色产品，广泛应用于新药研发、生命科学等科研项目；
•   提供虚拟筛选，离子通道筛选，代谢组学分析检测分析，药物筛选等专业技术服务；
•   设有专业的实验中心和严格的质控、验证体系；
•   提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；
•   产品的生物活性多经各国客户实验验证；
•   Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；
•   专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；
•   与世界各大制药公司及知名科研机构建立了长期的合作。