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MCE 国际站:CD40L/CD154/TRAP Protein, Human
CD40L (CD154; TRAP) 是 CD40 (TNFRSF5) 的配体,在 T 细胞中产生共刺激信号而发挥上调 IL-4 合成的作用。CD40L 在活化的 CD4+ T 淋巴细胞上特异性表达,参与 NF-κB/MAPK 信号通路。CD40L 还参与 B 细胞的分化、成熟和凋亡。人 CD40L 蛋白可裂解为 2 种亚型,包括膜状肽 (1-261 a.a.) 和可溶性肽 (113-261 a.a.) (作为整合素的配体)。重组人 CD40L/CD154/TRAP 蛋白 全长 149 个氨基酸 (M113-L261),由 E. coli 细胞表达,不带标签。
Synonyms: rHuCD40L; CD154; TRAP; TNFSF5; CD40LG; CD40-L
Species: Human
Source: E. coli
Biological Activity: 1. Measured in a cell proliferation assay using human B cells (Ramos) in the presence of 10 ng/mL Human IL-4. The ED50 this effect is 2.92 ng/mL, corresponding to a specific activity is 3.4247×105 units/mg.
2.The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be <20 μg/mL.
研究背景:"CD40 Ligand (CD40L; CD154; TRAP) belongs to the tumor necrosis factor (TNF) family, is the ligand for CD40/TNFRSF5, specifically expressed on activated CD4+ T-lymphocytes.
CD40L is a type II transmembrane protein on B cells triggers important signals for B cell differentiation, maturation, and apoptosis.
CD40L acts function by cross-linking on T-cells to generate a costimulatory signal and thus enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation, as well as promoting the production of interferon-γ, and TNF-α.
CD40L, binding with CD40 on antigen-presenting cells (APC), activates TNFR-associated factor 2- and IKK2-dependent pathways with stimulating I-κB kinase (IKK), increasing NF-κB DNA binding, and p65 nuclear translocation. The activation of I-κB kinase leads to strongly c-Jun N-terminal kinase activation as well as GST-I-κB and GST-p65 phosphorylation.
CD40L involves in MAPK pathways that strongly repress Bcl-6 with inducing the phosphorylation of Erk1/2, p38 and Jnk1/2 and activating IRF4 mediated by NF-κB.
CD40L also binds to and signals through several integrins, including αvβ3 and α5β1, which bind to the trimeric interface of CD40L. CD40L plays a major role in immune response and is a major target for inflammation.
CD40L is widely found in different animals, while the the sequence in Human is highly similar to Rhesus macaque (98.08%), but very different from Rat and Mouse with similarities of 77.31% and 77.69%, respectively. CD40L in Human is cleaved into 2 chains of membrane form (1-261 a.a.) and soluble form (113-261 a.a.), while the soluble form derives from the membrane form by proteolytic processing. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
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蛋白编号:P29965 (M113-L261)
基因 ID:959
产品分类:细胞因子和生长因子 | 肿瘤坏死因子超家族 | 肿瘤坏死因子配体 | CD40L 蛋白 | CD 抗原 | T 细胞 CD 蛋白 | CD40 Ligand/CD154 | CD 抗原 | 巨噬细胞 CD 蛋白 | CD40 Ligand/CD154 | 免疫检查点蛋白 | 刺激性免疫检查点
Categories:Cytokines and Growth Factors | TNF Superfamily | TNF Superfamily Ligands | CD40 Ligand | CD Antigens | T Cell CD Proteins | CD40 Ligand/CD154 | CD Antigens | Macrophage CD Proteins | CD40 Ligand/CD154 | Immune Checkpoint Proteins | Stimulatory Immune Checkpoint Molecules
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