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MCE 国际站:IL-27 Protein, Mouse (228a.a, HEK293, His)
IL-27 (Interleukin-27) 是 IL-12 家族的异二聚体细胞因子,由 EBI3 (Epstein-Barr-virus-induced 分子 3) 和 p28 两个亚基组成。IL-27增强CD8+ T 细胞的发育、增殖和细胞毒活性,从而间接促进抗肿瘤免疫。IL-27 Protein, Mouse (228a.a, HEK293, His) 是一种重组蛋白,C 端带有 His 标签,由两个亚基 EBI3 (IL27B) (228 个氨基酸 (M1-P228)) 和 IL27A (205 个氨基酸 (F29-S234)) 组成,在 HEK293 细胞中表达。
Synonyms: Interleukin-27 subunit beta; IL-27B; Ebi3; Interleukin-27 subunit alpha; IL-27A; p28
Species: Mouse
Source: HEK293
Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/mL (100 μl/well) can bind human IL27RA-Fc and the EC50 is 0.26-0.62 μg/mL. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is 2-10ng/mL.
研究背景:IL-27 is mainly produced by cells of myeloid origin such as monocytes, macrophages, dendritic cells, and microglial cells, in response to stimuli acting through Toll-like receptors or TNF-R-family members.
The amino acid sequence of human IL-27 protein has low homology for mouse IL-27 protein.
IL-27 acts through a heterodimer receptor consisting of IL-27Rα (WSX1) and gp130 chains, activates the JAK/STAT signaling pathway, which mainly involves STAT1 and STAT3 phosphorylation. STAT3 activation by IL-27 induces the expression of SOCS3, which inhibits further IL-27 signaling in a negative feedback loop, through inhibition of JAK activity.
IL-27 plays multiple roles in proinflammatory and anti-inflammatory immune responses. IL-27 enhances NF-κB/AP-1 activity and increases IL-12p40, TNF-α, and IL-6 production, increases TLR4 and TLR5 expression. IL-27 exerts inhibitory effects upon hPMSC adherence and proliferation, and promotes migration of hPMSCs. Besides, IL-27 can upregulate PDL1 expression and enhance the regulatory effects of hPMSCs on Th1 and Th2 cell differentiations and IL-10 secretion from CD4+T cells. IL-27 induces the proliferation and differentiation in hematopoietic stem cells. IL-27 increases IL-10 levels and the expression of p-STAT1, p-STAT3. IL-27 promotes the development and differentiation of CD4+IL-10+ T cells.
蛋白编号:O35228 (Y19-P228) &GSGSGGSGGSGSGKL& Q8K3I6 (F29-S234)
基因 ID:50498&246779
产品分类:细胞因子和生长因子 | 白细胞介素 | 白细胞介素-27
Categories:Cytokines and Growth Factors | Interleukin & Receptors | IL-27
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