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Animal-Free LIGHT/TNFSF14 Protein, Mouse (His)

MCE 国际站：Animal-Free LIGHT/TNFSF14 Protein, Mouse (His)

LIGHT蛋白 (CD258; TNFRSF14) 是由活化 T 细胞产生的 II 型跨膜蛋白，是 TNFRSF14/HVEM 配体，属于肿瘤坏死因子 (TNF) 家族。LIGHT 蛋白主要在脾脏中表达，具有膜结合型和可溶性型两种亚型。LIGHT蛋白可以作为肿瘤的免疫检查点分子，并刺激自然杀伤细胞产生干扰素 TFNγ，触发肿瘤凋亡信号。LIGHT 蛋白还参与 LTβR-NIK-p52 NF-κB 的显性通路促进炎症基因表达。此外，LIGHT 蛋白是激活淋巴样细胞的共刺激因子，对疱疹病毒感染有抑制作用。小鼠 LIGHT/TNFSF14 蛋白含有 239 个氨基酸和一个跨膜结构域 (38-58 a.a.)。无动物源，重组小鼠 LIGHT/TNFSF14 蛋白 (His) 是小鼠 LIGHT/TNFSF14 蛋白的胞外部分 (R58-V239)，由 E.coli 大肠杆菌表达，具有 C 端 His 标记。

Synonyms: Tumor necrosis factor ligand superfamily member 14; TNFSF14; HVEM-L; LIGHT  

Species: Mouse

Source: E. coli

Biological Activity: Measure by its ability to induce cytotoxicity in HT-29 cells. The ED₅₀ for this effect is <2 μg/mL.

研究背景：LIGHT/TNFSF14 is a type II transmembrane protein produced by activated T cells, belongs to tumor necrosis factor (TNF) family. LIGHT/TNFSF14 is a TNFRSF14/HVEM (herpesvirus entry mediator) ligand, engages the receptor for the LTalphabeta heterotrimer but does not form complexes with either secreted lymphotoxin alpha (LTalpha) or LTbeta.
LIGHT/TNFSF14 is predominantly expressed in the spleen but also found in the brain. It is weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
LIGHT/TNFSF14 has a transmemberane, thus it can be leaved into 2 chains: membrane form and soluble form. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
In tumor immunology, TNFSF14/LIGHT also serves as a novel immune checkpoint molecule for glioblastoma multiforme (GBM), as well as lung carcinoma, breast carcinoma, cervical cancer, and prostate cancer. TNFSF14/LIGHT can stimulate NK cells to produce IFNγ via nuclear factor-κB (NFκB) RelA/p50 signaling. TNFSF14/LIGHT sustains the function of CD8⁺ effector T cells, trigger apoptosis of various tumor cells.
In cell signaling, TNFSF14/LIGHT binds to lymphotoxin-β receptor (LTβR) and HVEM for activating both of them, and disrupts the HVEM-BTLA complex in surface-bound form, and facilitates HVEM-BTLA complex formation in the soluble form.
TNFSF14/LIGHT promotes an inflammatory esophageal fibroblast in vitro via a LTβR-NIK-p52 NF-κB dominant pathway with promoting inflammatory gene expression and down-regulating homeostatic factors including WNTs, BMPs and type 3 semaphorins.
Beside that, TNFSF14/LIGHT protein is a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. TNFSF14/LIGHT also prevents tumor necrosis factor alpha mediated apoptosis in primary hepatocyte.

蛋白编号：Q9QYH9 (R58-V239)

基因 ID：50930

产品分类：细胞因子和生长因子  |  肿瘤坏死因子超家族  |  肿瘤坏死因子配体  |  LIGHT 蛋白  |  无动物成分重组蛋白

Categories：Cytokines and Growth Factors  |  TNF Superfamily  |  TNF Superfamily Ligands  |  LIGHT Proteins  |  Animal-free Recombinant Proteins

货号: HY-P700221AF

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