MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
CAS No. : 4091-99-0
MCE 国际站:H2DCFDA
产品活性:H2DCFDA (DCFH-DA) 是一种可渗透细胞的,用于检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)。
研究领域:Immunology/Inflammation | NF-κB | Metabolic Enzyme/Protease
作用靶点:Reactive Oxygen Species
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
?1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
?2. Cells are incubated with 5 μM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
?3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA.
相关产品:D-α-Hydroxyglutaric acid disodium | 8α-Tigloyloxyhirsutinolide 13-O-acetate | Anticancer agent 120 | 7-Hydroxy-4-methyl-2(1H)-quinolone | N-tert-Butyl-α-phenylnitrone-d14 | BDP 581/591 NHS ester | Cysteamine-d4 hydrochloride | L-Ascorbic acid sodium salt | Manganese(salen) chloride | Hydroxyphenyl Fluorescein | Glucosamine sulfate | Maresin 1 | Berberine | 1,3-Dicaffeoylquinic acid | Ciprofloxacin | Echinocystic acid | Lacto-N-fucopentaose I | Nisoldipine-d6 | Arg-Tyr | HKPerox-2 | 3-Indolepropionic acid | 2-Methoxyestradiol-13C,d3 | Albendazole | QD394 | AKBA | Cedrin | Deferoxamine | Ellagic acid | Acetylcysteine-d3 | N-Acetyl-D-cysteine
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物 | 寡核苷酸 | 抗体 | 点击化学
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Fluorescent Dye | PROTAC | Isotope-Labeled Compounds | Oligonucleotides | Antibodies | Click Chemistry
货号: HY-D0940
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;
• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。