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5(6)-CFDA

CAS No. : 124387-19-5

MCE 国际站：5(6)-CFDA

产品活性：5(6)-CFDA 是一种常见的脂肪族荧光素系列物。CFDA 可在细胞内自由扩散，并被细胞内非特异性脂肪酶水解生成羧基荧光素 (CF)。与荧光素染料相比，含有 CF 的部分带有额外的负电荷，使它能够更好地保留在细胞中。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Preparation of 5(6)-CFDA working solution
1. Preparation of the stock solution
Dissolve 1mg 5(6)-CFDA in 0.21 mL DMSO to obtain 10 mM of 5(6)-CFDA.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Preparation of 5(6)-CFDA working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of 5(6)-CFDA working solution.
Note: Please adjust the concentration of 5(6)-CFDA working solution according to the actual situation.

Cell staining
1. Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2. Add 1 mL of 5(6)-CFDA working solution, and then incubate at room temperature for 30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4. Wash twice with PBS, 5 minutes each time.
5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of 5(6)-CFDA working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

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