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Fluo-3AM

CAS No. : 121714-22-5

MCE 国际站：Fluo-3AM

产品活性：Fluo-3 AM 是 Ca²⁺ 的荧光螯合剂，对钙有很高的亲和性。Fluo-3 AM 可对细胞内钙离子进行特异性识别，具有高灵敏性，对细胞毒性较低，增加 AM乙酰甲酯可以好的进入到细胞内，在被细胞内的酯酶剪切后滞留在细胞内与钙离子结合，产生较强荧光。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: 1. Preparation of Fluo-3 AM working solution
1.1 Preparation of the stock solution
Dissolve 1 mg Fluo-3 AM in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Fluo-3 AM working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Fluo-3 AMworking solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×10⁶/mL
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
Storage
-20℃, 1 year
Protect from light

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