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1,3-Diphenylisobenzofuran

CAS No. : 5471-63-6

MCE 国际站：1,3-Diphenylisobenzofuran

产品活性：1,3-Diphenylisobenzofuran (DPBF) 作为一种选择性探针，用于检测和定量测定含有不同活性氮和氧物种 (RNOS) 样品中的过氧化氢。DPBF是一种荧光探针，近 20 年来，人们认为它与一些活性氧 (如单重态氧和羟基、烷基氧或烷基过氧自由基) 发生高度特异的反应。

研究领域：Others  |  Immunology/Inflammation  |  NF-κB  |  Metabolic Enzyme/Protease

作用靶点：Fluorescent Dye  |  Reactive Oxygen Species

In Vitro: General Protocol

Preparation of DPBF working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of DPBF in 3.6992 mL of DMSO to obtain 10 mM of DPBF.
Note: It is recommended to store the stock solution at -20°C, -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DPBF working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 20 μM of DPBF working solution.
Note: Please adjust the concentration of DPBF working solution according to the actual situation.

Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of DPBF working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of DPBF working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

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