MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
CAS No. : 548-62-9
MCE 国际站:Crystal Violet
产品活性:Crystal Violet,也称为龙胆紫、甲基紫 10B,是一种三苯甲烷类染料,碱性染料,可以和细胞核中的 DNA 结合,从而将细胞核染为深紫色。常用于革兰氏染色来进行细菌分类,或者用于细胞或组织学染色。
研究领域:Others | Anti-infection
作用靶点:Fluorescent Dye | Influenza Virus | Bacterial
In Vitro: General Protocol
1.Preparation of MitoSOX Red working solution
Dilute the 2 g Crystal Violet in 20 mL 95% ethanol andmixed with 80 ml 1% ammonium oxalate solution solution for 24 h to obtain working solution.
2.Sample processing
2.1 Paraffin section:
First dewaxing with xylene for 5-10 min, then with fresh xylene, then dewaxing with 100% ethanol for 5 min after dewaxing for 5-10 min. Then 90% ethanol was used for 2 min. At last, 70% ethanol was used for 2 min. DDH2O 2 min.
2.2 Frozen section:
distilled water for 2 min.
2.3 Tissue culture cells:
fixed with 4% paraformaldehyde for more than 10 min. Wash with distilled water for 2 min, replace with fresh distilled water, and wash again for 2 min.
Note: Other fixatives can be selected according to specific experiments.
3.Crystal violet staining
3.1 After the sample to be tested is treated with the above method, the crystal violet dyeing solution is directly added and dyed for 10 minutes at room temperature (the dyeing solution needs to cover the sample, and the specific dyeing time can be adjusted according to the required dyeing results).
3.2 Wash well in distilled water.
3.3 Dry at room temperature.
3.4 Observe and photograph by light microscope.
Storage
4°C, 2 year
Protect from light
Precautions
1. It is recommended to take 1-2 samples for pre-test when using this product for the first time.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
相关产品:Myricetin 3-O-α-L-arabinopyranoside | MeOSuc-Ala-Ala-Pro-Val-pNA | BDP 558/568 amine | Ganoderic acid TR | (1R,2S,7R)-Sitafloxacin-d4 hydrochloride | Nebacumab | AC-green | Naphthalene-1,2-dicarbaldehyde | Gepotidacin | Rhodamine 800 | Amycolatopsin A | Antibiotic A-33853 | Estradiol hemihydrate | Sulbactam-d2 sodium | Vari Fluor 532 SE | Antimicrobial agent-5 | NBTIs-IN-4 | Proanthocyanidins | Acridine Orange hydrochloride | Anticancer agent 120 | Azlocillin sodium salt | Antitubercular agent-13 | N-(7-Nitrobenzofurazan-4-yl)phallacidin | Lenampicillin hydrochloride | Biotin-11-UTP | BODIPY FL SSE | PC58538 | SPR206 acetate | Caracemide | Antimicrobial agent-10
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物 | 寡核苷酸 | 抗体 | 点击化学
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Fluorescent Dye | PROTAC | Isotope-Labeled Compounds | Oligonucleotides | Antibodies | Click Chemistry
货号: HY-B0324A
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;
• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。