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Green DND-26

CAS No. : 220524-71-0

MCE 国际站：Green DND-26

产品活性：Green DND-26 为绿色荧光标记的溶酶体探针，具有 504/511 nm 的最大激发/发射波长。结构上由一个荧光基团和相连的弱碱基构成，可自由穿过细胞膜，一般聚集在球形细胞器上，适用于观察溶酶体内部生物合成及相关发病机理。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Preparation of Green DND-26 working solution
1. Preparation of the stock solution
1.1 Restore the solution to the room temperature and concentrate it to the bottom of the tube by instantaneous centrifugation.
1.2 Dilute 1 mM storage solution to the working solution using medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM.
Note: Please adjust the concentration of Green DND-26 working solution according to the actual situation, and use it now.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Green DND-26 working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃ save, Protect from light

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