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CAS No. : 231946-72-8
MCE 国际站:LysoTracker Red
产品活性:LysoTracker Red 为红色荧光标记的溶酶体探针,具有 577/590 nm 的最大激发/发射波长。结构上由一个荧光基团和相连的弱碱基构成,可自由穿过细胞膜,一般聚集在球形细胞器上,适用于观察溶酶体内部生物合成及相关发病机理。
研究领域:Others
作用靶点:Biochemical Assay Reagents | Fluorescent Dye
In Vitro: Preparation of LysoTracker Red working solution
1. Working fluid configuration:
1.1 Restore the solution to room temperature and concentrate it at the bottom of the tube by instantaneous centrifugation.
1.2 Dilute 1 mM storage solution to the working solution using a medium or appropriate buffer (such as PBS) . The recommended working solution is 50-100 nM;
Note: Please adjust the concentration of the LysoTracker Red working solution according to the actual situation, and use it now.
2. Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of LysoTracker Red working solution, and then incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
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