MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务。
CAS No. : 5578-73-4
MCE 国际站:Sanguinarine chloride
产品活性:Sanguinarine (Sanguinarin) chloride 是一种来源于 Sanguinaria Canadensis 的生物碱,可通过激活活性氧 (ROS) 的产生来刺激细胞凋亡。Sanguinarine 诱导的细胞凋亡与 JNK 和 NF-κB 的活化有关。
研究领域:Apoptosis | Autophagy | Anti-infection
作用靶点:Apoptosis | Autophagy | Bacterial | Parasite
结构分类:生物碱 | 吡啶类生物碱 | 结构分类
来源:植物 | 罂粟科 | 血根草
In Vitro: Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability, 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h, and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile, the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA, which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner, indicating increased caspase-3 activity stimulated by Sanguinarine.
In Vivo: To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo, 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24, 48 and 72 h after treatment, bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment, the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors, the group treated with Sanguinarine exhibits significantly more cell apoptosis, indicated by the increased green signals from the sporadic apoptotic cells.
相关产品:Natural Product Library Plus | Drug Repurposing Compound Library Plus | FDA-Approved Drug Library Plus | FDA-Approved Drug Library Mini | Bioactive Compound Library Plus | Anti-Infection Compound Library | Apoptosis Compound Library | NF-κB Signaling Compound Library | Natural Product Library | FDA-Approved Drug Library | Anti-Cancer Compound Library | Autophagy Compound Library | Drug Repurposing Compound Library | Antibacterial Compound Library | Medicine Food Homology Compound Library | Traditional Chinese Medicine Active Compound Library | FDA Approved & Pharmacopeial Drug Library | Alkaloids Library | Antiparasitic Compound library | Anti-Cancer Natural Product Library | Plant-Sourced Natural Product Library | FDA-Approved Anticancer Drug Library | Human Metabolite Library | Heterocyclic Compound Library | Off-patent Drug Library | SY-5609 | Antifungal agent 26 | Myricetin 3-O-α-L-arabinopyranoside | BRD4 Inhibitor-18 | 8α-Tigloyloxyhirsutinolide 13-O-acetate | (1R,2S,7R)-Sitafloxacin-d4 hydrochloride | Quinidine-d3 | Nebacumab | Angulatin G | Acridine Orange base | N-Desethyl amodiaquine-d5 | Gepotidacin | Amycolatopsin A | Palitantin | Antibiotic A-33853 | Estradiol hemihydrate | AAPK-25 | Sulbactam-d2 sodium | Dutasteride-13C6 | Oleic acid-13C | Antimicrobial agent-5 | Proguanil-d6 | NBTIs-IN-4 | Proanthocyanidins | Anticancer agent 120 | Azlocillin sodium salt | Abacavir sulfate | Antitubercular agent-13 | Meloxicam-d3-1
热门产品线:重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物 | 寡核苷酸 | 抗体 | 点击化学
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Fluorescent Dye | PROTAC | Isotope-Labeled Compounds | Oligonucleotides | Antibodies | Click Chemistry
货号: HY-N0052A
品牌介绍:
• MCE (MedChemExpress) 拥有200 多种全球独家化合物库,我们致力于为全球科研客户提供前沿最全的高品质小分子活性化合物;
• 50,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领;
• 产品种类涵盖各种重组蛋白,多肽,常用试剂盒 ,更有 PROTAC、ADC 等特色产品,广泛应用于新药研发、生命科学等科研项目;
• 提供虚拟筛选,离子通道筛选,代谢组学分析检测分析,药物筛选等专业技术服务;
• 设有专业的实验中心和严格的质控、验证体系;
• 提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告,确保产品的高纯度、高品质;
• 产品的生物活性多经各国客户实验验证;
• Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果;
• 专业团队跟踪最新的制药及生命科学研究进展,为您提供全球最新的活性化合物;
• 与世界各大制药公司及知名科研机构建立了长期的合作。