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CFDA-SE
CAS No. : 150347-59-4
MCE 国际站:CFDA-SE
产品活性:CFDA-SE 是一种可穿透细胞膜的荧光染料,其能与胞内细胞骨架蛋白中的游离胺基反应,最终形成具有荧光的蛋白复合物。该染料进入细胞后主要定位于细胞膜、细胞质和细胞核,在细胞核的染色效果最强。CFDA-SE 染料可用于检测细胞增殖,CFDA-SE 可随着细胞的分裂增殖而被子代细胞均匀继承,其含量的衰减与细胞分裂次数成正比,该荧光信号可在 488 nm 的激发光下进行检测,并可通过流式细胞仪进行分析。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Preparation of CFDA-SE working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of CFDA-SE in 0.1794 mL of DMSO to obtain 10 mM of CFSE.
Note: It is recommended to store the stock solution at -20 ℃ or -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of CFDA-SE working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of CFDA-SE working solution.
Note: Please adjust the concentration of CFDA-SE working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of CFDA-SE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
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热门产品线:重组蛋白 | 药物筛选 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products:Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
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