Rose-β-D-Gal 产品活性：Rose-β-D-Gal 是荧光染料，也是 β-半乳糖苷酶底物。Rose-β-D-Gal 在反应后产生粉红色/品红的颜色，可用于检测 β-gal 活性。 产品来源： https://www.medchemexpress.cn/rose-β-d-gal.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Immunostaining of Tissues: 1.Prepare 25 mg/mL Rose-β-D-Gal stock solution in Dimethyl Sulfoxide (DMSO) and store at -20 °C. It is vital to protect the stock solution from the light. 2.Prepare rinse buffer for Rose-β-D-Gal staining: 0.1 % Sodium Deoxycholate, 0.2 % IGEPAL CA-630, 2 mM MgCl2 in 0.1 M Na Phosphate buffer (pH 7.3). Prepare 1,000 mL of 0.5 M Na Phosphate buffer (pH 7.3) by mixing with 158 mL of 1 M NaH2PO4, 342 mL of 1 M Na 2HPO4, and 500 mL water. 3.Prepare substrate solution for Rose-β-D-Gal fresh, containing 1 mg/mL Rose-β-D-Gal, 5 mM Potassium Ferricyanide, and 5 mM Potassium Ferrocyanide in the rinse buffer. 4.Rehydrate frozen tissue sections in PBS: quickly wash with PBS twice, wash with rinse buffer for 10 min 3 time. 5.Expose to β-galactosidase substrate Rose-β-D-Gal: stain at 37 ℃ for as long as needed to see stain, up to an overnight time period, keep covered and in the dark during color development. Wash with PBS for 5 min twice. 6.Fix tissue in 4% PFA, unless on intend to continue with in situ hybridizations, and continue to step 4. Note: Rose-β-D-Gal is not very stable in alcohols or organic solvents, so must use water-based counterstains such as Gill’s hematoxylin and cover slipping with aqueous mountant. 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。