耗材描述
4-Di-1-ASP
产品活性:4-Di-1-ASP 是一种苯乙烯基染料,可用作活脑组织中的神经胶质瘤细胞染色,用于细胞结构、活力、增殖及内吞、胞吐、吞噬作用分析,也可用于观察活细胞线粒体结构。4-Di-1-ASP 在显微镜成像时可发出绿色荧光 (λex /λem = 475/606 nm)。
产品来源: https://www.medchemexpress.cn/4-di-1-asp.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Dissolve the dye in water and configure a mother liquor at a concentration of 2 mM.
2.Place the prepared cell sections in an incubator at 35°C, add the prepared dye solution to a final concentration of 1 μM and incubate with the stain for 10 min.
3.The prepared stained cell sections are placed on a microscope slide, cover with a coverslip and observe using a fluorescent confocal microscope with a maximum excitation wavelength of 475 nm and a maximum emission wavelength of 606 nm.
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