BODIPY 493/503 产品活性：BODIPY493/503 是一种 BODIPY 染料。BODIPY 染料是一种具有强紫外吸收能力的小分子染料，其荧光峰比较尖锐，量子产率高。其对环境的极性和 pH 值相对不敏感，因此，在不同生理条件下相对稳定。由于其结构的非对称性，BODIPY 具有多种衍生结构产物。BODIPY 脂滴类染料可以很好的穿过细胞膜进入细胞内部，通过在细胞内的极性脂类上定位以进行特异性染色，因此，该染料可用于标记活细胞和固定细胞。最大激发/发射波长：493/503 nm。 产品来源： https://www.medchemexpress.cn/bodipy-493-503.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: General Protocol 1. Preparation of BODIPY 493/503 working solution 1.1 Preparation of the stock solution Dissolve 1 mg BODIPY 493/503 in 382 μL DMSO to obtain 10 mM of stock solution. Note: It is recommended to store the stock solution at -20°C or -80°C, keep away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of BODIPY 493/503 working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution. Note: Please adjust the concentration of BODIPY 493/503 working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a. Centrifuge at 1000 g at 4°C for 3-5 min and then discard the supernatant. Wash twice with PBS, 5 min each time.The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 min. c.Centrifuge at 400 g at 4°C for 3-4 min and then discard the supernatant. d. Wash twice with PBS, 5 min each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 min. d. Wash twice with medium, 5 min each time. Observation by fluorescence microscopy or flow cytometry. Storage -20°C, 1 year; Protect from light. Precautions 1. Please adjust the concentration of BODIPY 493/503 working solution according to the actual situation. 2. Experiments suggest a positive control, incubate the control group cells with 30 μM oleic acid for 8 h and then perform subsequent experiments. 3. This product is only for R&D use, not for drug, household, or others. 4. For your safety and health, please wear a lab coat and disposable gloves to operate. 相关产品：MitoSOX Red | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE | TMRM Perchlorate 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。