Boc-Val-Pro-Arg-AMC 产品活性：Boc-Val-Pro-Arg-MCA 是一种敏感的荧光底物，用于测定胰蛋白酶样丝氨酸蛋白酶 (trypsin-like serine proteases) 活性。 产品来源： https://www.medchemexpress.cn/boc-val-pro-arg-amc.html 研究领域：Others 作用靶点：Fluorescent Dye In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Trypsin activity assay 1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0). 2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-MCA. 3. Incubate at 55 °C for 10 min. 4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction. 5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm). Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 相关产品：MitoSOX Red | BODIPY 493/503 | D-Luciferin potassium | Nile Red | FITC | JC-1 | Propidium Iodide | DiR | FITC-Dextran (MW 4000) | Dihydroethidium | CFDA-SE | 2-NBDG | Calcein-AM | DiI | Thiazolyl Blue | Cy5.5 | TMRE | Fluo-4 AM | CY5 | Indocyanine green | PKH 26 | DiD perchlorate | Acridine Orange hydrochloride | BODIPY 581/591 C11 | FM4-64 | DiO | CY5-SE 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。