耗材描述
Dnp-PLGLWA-DArg-NH2 TFA
产品活性:Dnp-PLGLWA-DArg-NH2 TFA 是 MMP-1 和 MMP-9 的荧光底物。Dnp-PLGLWA-DArg-NH2 TFA 可用来定量 MMPs 的活性 (Ex=280 nm, Em=360 nm)。
产品来源: https://www.medchemexpress.cn/dnp-plglwa-darg-nh2-tfa.html
研究领域:Metabolic Enzyme/Protease
作用靶点:MMP
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Enzyme activity assays are performed in 50 mmol/l Tris-HCl buffer, pH 7.5, 0.15 mol/l NaCl, 10 mmol/l CaCl2, 0.02% TNC buffer containing 0.05% Brij 35 and 50 μM ZnSO4.
2. Dnp-PLGLWA-DArg-NH2 TFA. Each fraction is incubated with 1 μM substrate at 37°C for 20 h. 3. Stop the reaction by the addition of 3% acetic acid.
4. Measure the fluorescence using wavelengths of 280 nm (excitation) and 360 nm (emission) with a fluorescence reader.
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