Ac-WEHD-AFC TFA 产品活性：Ac-WEHD-AFC TFA 是一种荧光 caspase-1 底物。Ac-WEHD-AFC TFA 可检测 caspase-1 荧光活性，用于肿瘤和炎症的研究。 产品来源： https://www.medchemexpress.cn/ac-wehd-afc-tfa.html 研究领域：Apoptosis 作用靶点：Caspase In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Caspase-1 Fluorogenic Activity Assay: 1. Incubate the cells according to your normal protocol. 2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC TFA. 5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute. 相关产品：Bioactive Compound Library Plus | Apoptosis Compound Library | Immunology/Inflammation Compound Library | Anti-Cancer Compound Library | Pyroptosis Compound Library | Peptide Library | Membrane Protein-targeted Compound Library | Bioactive Compound Library Max | Z-VAD-FMK | Q-VD-OPh | Belnacasan | Sulforaphane | Z-DEVD-FMK | Emricasan | Tauroursodeoxycholate | Aspirin | Z-IETD-FMK | Ac-YVAD-cmk | Oxidopamine hydrobromide | Ac-DEVD-CHO | Raptinal | Chlorin e6 | Crustecdysone | Guggulsterone | Wedelolactone | Benzbromarone | Ac-FLTD-CMK | BOC-D-FMK | Fucoxanthin | Ginsenoside Rh2 | Dehydrocorydaline | VRT-043198 | Terfenadine | Digitoxin | PAC-1 品牌介绍: MCE (MedChemExpress) 拥有数百种全球独家化合物，我们致力于为全球科研客户提供最新最全的高品质小分子活性化合物；10,000 多种高选择性抑制剂、激动剂涉及各热门信号通路及疾病领域；设有专业的实验中心和严格的质控、验证体系；提供 LC/MS、NMR、HPLC、手性分析、元素分析等各项质检报告，确保产品的高纯度、高品质；产品的生物活性多经各国客户实验验证；Nature, Cell, Science 等多种顶级期刊及制药专利收录了MCE客户的科研成果；专业团队跟踪最新的制药及生命科学研究进展，为您提供全球最新的活性化合物；与世界各大制药公司及知名科研机构建立了长期的合作。