耗材描述
Ac-VDVAD-AFC
产品活性:Ac-VDVAD-AFC 是一种 caspase 特异性荧光底物。Ac-VDVAD-AFC 可检测 caspase-3-like 活性和 caspase-2 活性,用于肿瘤和癌症的研究。
产品来源: https://www.medchemexpress.cn/ac-vdvad-afc.html
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Culture cells in in a 6 well plate at a density of 3×105 cells per well or in a 96 well plate at a density of 3×104 cells per well. Incubate the cells according to your normal protocol.
2. 2 μM arsenic or cotreated with 2 μM arsenic and 25 μM quercetin to treate A549 cells for 24 h.
3. 50 μL of cell lysate is made up to 200 μL buffer containing HEPES 20 mM, 40 μM DEVD‐AMC, or Ac‐VDVAD‐AFC, and 2mM dithiothreitol.
4. 40 μM fluorescent substrate Ac-VDVAD-AFC is used to measure Caspase-2 activity for 4 days.
5. Fluorescence is measured at 460 and 505 nm wavelengths, respectively (The activity was calculated as arbitrary fluorescence units per min per mg protein).
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