耗材描述
Suc-AAPF-pNA
产品活性:Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) 是一种对硝基苯胺 (pNA) 显色底物,Km 为 1.7 mM。Suc-AAPF-pNA 的裂解可以释放出 4-硝基苯胺,其颜色为黄色,可以通过分光光度法进行测量。Suc-AAPF-pNA 可用于测量人中性粒细胞中游离和膜结合的组织蛋白酶 G。
产品来源: https://www.medchemexpress.cn/suc-aapf-pna.html
研究领域:Others
作用靶点:Others
In Vitro: The elastase activity is measured as activity against the substrate Suc-AAPF-Pna. Aliquots (10 μL) of SmCTF samples are incubated at 30 °C with 200 μM Suc-AAPF-pNA and the activity of cercarial elastase measured as abosorbance at 400 nm.
Proteolytic activity is measured by hydrolysis of the substrate suc-AAPF-pNA. Serial dilutions of the proteases are made in 100 mM Tris buffer, pH 8.6, containing 10 mM CaCl2. Enzyme activity is measured at 25°C in 100 mM Tris/HCl, pH 8.6 containing 1.6 mM suc-AAPF-pNA, 0.0005% Tween-80 and 1% DMSO by monitoring the absorbance change at 410 nm using a spectrophotometer. Each dilution of enzyme is tested in triplicate.
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